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tiliGLOW

tiliGLOW

€555.00
€555.00
Sale Sold out

tiliGLOW: one incubation, ultra-bright signal.

Who it's for

Researchers running immunofluorescence, IHC, ICC, flow cytometry or spatial assays who need to detect low-abundance targets — without amplification steps, and without changing their validated primary antibodies.

Key benefits

  • A single incubation delivers ultra-bright signal — no enzymatic amplification, no extra reagents.
  • Drop-in replacement for conventional secondary antibodies; your existing protocol stays unchanged.
  • Pre-mix with your primary antibody to run several rabbit primaries on one section without cross-talk.
  • Detects rare and low-abundance targets that stay invisible to standard dye-labelled IgGs.
  • Replaces Tyramide Signal Amplification (TSA) without the enzymatic step.

How to use

  1. Dilute to working concentration: 2 nM for ICC/IF, 5 nM for IHC, 2 nM for flow cytometry.
  2. Either add directly to the sample after your primary antibody, or pre-mix with the primary in a tube for multiplexing.
  3. Incubate in a single step — no separate amplification or secondary incubation.
  4. Wash and image using your standard imaging protocols.
Product description

tiliGLOW is an ultra-bright signal amplifier for high-sensitivity applications: a DNA nanostructure carrying a large number of dyes, coupled to a binding moiety — either streptavidin or an anti-Rabbit nanobody.

The nanostructure holds each dye at a defined distance from its neighbours, preventing self-quenching, and is kept as compact as possible to preserve tissue penetration and diffusion. Because the anti-Rabbit binding moiety is monovalent, it cannot cross-link primary antibodies into lattices — which is what makes pre-mixing, and therefore same-species multiplexing, possible.

Ruggedized variants are modified for extended stability in harsher conditions, such as low-salt buffers or samples containing significant DNase activity.

For Research Use Only (RUO). Not for diagnostic use.

Specifications
Format DNA nanostructure carrying multiple dyes, coupled to a binding moiety
Scale 10 pmol (200 µL at 50 nM)
Working concentration ICC/IF 2 nM (1–5 nM) · IHC 5 nM (3–10 nM) · Flow cytometry 2 nM (1–3 nM)
Shipping Ambient temperature
Storage Store at 2–8 °C on receipt. Protect from light. See product data sheet for stability.
Regulatory For Research Use Only (RUO). Not for diagnostic use.
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