Single-molecule placement

Random loading leaves some molecules too close together and many small wells empty or multiply occupied. DNA nanostructures make placement part of the design: control capture-site spacing on a surface, or develop a carrier that fits an individual nanowell.

Defined patterns on glass

DNA origami positions capture handles on an anti-fouling surface. tiliGRID examples use four sites separated by 40 nm; distinct DNA handles can combine different molecular functions in one pattern. After imprinting, the origami is removed and the attachment sites remain.

Spacing that separates signals

A designed nanostructure footprint helps keep neighboring signals distinguishable while making better use of the imaging area. We adapt the footprint, capture chemistry and deposition to the spacing your optical measurement needs.

One carrier per nanowell

For chips with many small holes, a carrier sized to the well can favor single occupancy and position a molecular attachment within the detection volume. We develop the carrier, payload attachment and loading process around your well dimensions, surface chemistry and readout.

Develop your placement workflow

Tell us your molecule, substrate, desired spacing and measurement method. Discuss a placement project.

Explore tiliGRID Biotin, custom DNA designs or microscopy references.