Available scales:

• 0.5 ml at 400 nM. 200 picomoles (467 micrograms) of single-stranded, circular DNA. The scaffold is provided at a high concentration to enable mixing of multiple different scaffold sequences.

The orthogonal scaffold strand sequence is based on the sequences described in the following publication:
Custom-Size, Functional, and Durable DNA Origami with Design-Specific Scaffolds

There, the orthogonal nature of the scaffold strand sequences is used to, for example, assemble multiple separate nanostructures in a single one-pot reaction, or to assemble larger nanostructures containing multiple different scaffold strands, again in a one-pot reaction.

The scaffold strand that we offer is identical to the "CS4" sequence from the publication, up to 4 bases that differ based on our post-production sequencing results. It has a length of 7557 bases. One substitution at position 666 (C → G), a deletion at position 2953 (T) and a double deletion at position 5813-5814 (AG).

DNA Sequence

For details and references see our product data sheets (pdf):

Product data sheet 0.5 mL at 400 nM