Correlative light and electron microscopy

Electron microscopy reveals molecular surroundings, but identifying a particular target can be difficult. tiliPOINTER combines fluorescent dyes and gold nanoparticles in one targeted label, connecting optical identification with electron and cryo-electron microscopy.

Find the target in its context

Fluorescence helps locate a region of interest; gold supplies electron contrast. The three-particle format adds a recognizable row oriented towards the binding end, while the one-particle format provides a simpler label geometry.

Connect labeling with cryo-EM and cryo-ET

Develop workflows for isolated complexes on grids, accessible cell-surface targets or exposed targets in sections. The same design principle supports correlative light and electron microscopy and target identification in cryo-electron tomography.

Choose the signal and binder together

Standard reagents combine AF647, anti-rabbit binding and one or three 10 nm gold particles. Custom formats adapt the dye, binder or particle arrangement to your sample and readout. Discuss your imaging workflow.