{"product_id":"tiliglow","title":"tiliGLOW","description":"\u003ch3\u003e\n\u003cstrong\u003e\u003cspan style=\"color: rgb(141, 175, 67);\"\u003etiliGLOW:\u003c\/span\u003e\u003c\/strong\u003e one incubation, ultra-bright signal.\u003c\/h3\u003e\n\u003ch3\u003eWho it's for\u003c\/h3\u003e\n\u003cp\u003eResearchers running immunofluorescence, IHC, ICC, flow cytometry or spatial assays who need to detect low-abundance targets — without amplification steps, and without changing their validated primary antibodies.\u003c\/p\u003e\n\u003ch3\u003eKey benefits\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eA single incubation delivers ultra-bright signal — no enzymatic amplification, no extra reagents.\u003c\/li\u003e\n\u003cli\u003eDrop-in replacement for conventional secondary antibodies; your existing protocol stays unchanged.\u003c\/li\u003e\n\u003cli\u003ePre-mix with your primary antibody to run several rabbit primaries on one section without cross-talk.\u003c\/li\u003e\n\u003cli\u003eDetects rare and low-abundance targets that stay invisible to standard dye-labelled IgGs.\u003c\/li\u003e\n\u003cli\u003eReplaces Tyramide Signal Amplification (TSA) without the enzymatic step.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eHow to use\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eDilute to working concentration: 2 nM for ICC\/IF, 5 nM for IHC, 2 nM for flow cytometry.\u003c\/li\u003e\n\u003cli\u003eEither add directly to the sample after your primary antibody, or pre-mix with the primary in a tube for multiplexing.\u003c\/li\u003e\n\u003cli\u003eIncubate in a single step — no separate amplification or secondary incubation.\u003c\/li\u003e\n\u003cli\u003eWash and image using your standard imaging protocols.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv class=\"accordion\"\u003e\n\u003cdetails open=\"\"\u003e\n\u003csummary\u003eProduct description\u003cspan class=\"icon-caret\"\u003e\u003csvg viewbox=\"0 0 10 6\" class=\"icon icon-caret\"\u003e\u003cpath clip-rule=\"evenodd\" d=\"M9.354.646a.5.5 0 0 0-.708 0L5 4.293 1.354.646a.5.5 0 0 0-.708.708l4 4a.5.5 0 0 0 .708 0l4-4a.5.5 0 0 0 0-.708\" fill-rule=\"evenodd\" fill=\"currentColor\"\u003e\u003c\/path\u003e\u003c\/svg\u003e\u003c\/span\u003e\u003c\/summary\u003e\n\u003cdiv class=\"accordion__content\"\u003e\n\u003cp\u003e\u003cstrong\u003etiliGLOW\u003c\/strong\u003e is an ultra-bright signal amplifier for high-sensitivity applications: a DNA nanostructure carrying a large number of dyes, coupled to a binding moiety — either streptavidin or an anti-Rabbit nanobody.\u003c\/p\u003e\n\u003cp\u003eThe nanostructure holds each dye at a defined distance from its neighbours, preventing self-quenching, and is kept as compact as possible to preserve tissue penetration and diffusion. Because the anti-Rabbit binding moiety is monovalent, it cannot cross-link primary antibodies into lattices — which is what makes pre-mixing, and therefore same-species multiplexing, possible.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRuggedized\u003c\/strong\u003e variants are modified for extended stability in harsher conditions, such as low-salt buffers or samples containing significant DNase activity.\u003c\/p\u003e\n\u003cp\u003eFor Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion\"\u003e\n\u003cdetails open=\"\"\u003e\n\u003csummary\u003eSpecifications\u003cspan class=\"icon-caret\"\u003e\u003csvg viewbox=\"0 0 10 6\" class=\"icon icon-caret\"\u003e\u003cpath clip-rule=\"evenodd\" d=\"M9.354.646a.5.5 0 0 0-.708 0L5 4.293 1.354.646a.5.5 0 0 0-.708.708l4 4a.5.5 0 0 0 .708 0l4-4a.5.5 0 0 0 0-.708\" fill-rule=\"evenodd\" fill=\"currentColor\"\u003e\u003c\/path\u003e\u003c\/svg\u003e\u003c\/span\u003e\u003c\/summary\u003e\n\u003cdiv class=\"accordion__content\"\u003e\n\u003ctable style=\"width: 100.087%; border-collapse: collapse; height: 293.906px;\" cellspacing=\"0\" cellpadding=\"8\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003cth style=\"text-align: left; width: 39.9652%; height: 39.1875px;\"\u003eFormat\u003c\/th\u003e\n\u003ctd style=\"width: 59.9479%; height: 39.1875px;\"\u003eDNA nanostructure carrying multiple dyes, coupled to a binding moiety\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"text-align: left; width: 39.9652%; height: 19.5938px;\"\u003eScale\u003c\/th\u003e\n\u003ctd style=\"width: 59.9479%; height: 19.5938px;\"\u003e10 pmol (200 µL at 50 nM)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003cth style=\"text-align: left; width: 39.9652%; height: 39.1875px;\"\u003eWorking concentration\u003c\/th\u003e\n\u003ctd style=\"width: 59.9479%; height: 39.1875px;\"\u003eICC\/IF 2 nM (1–5 nM) · IHC 5 nM (3–10 nM) · Flow cytometry 2 nM (1–3 nM)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 19.5938px;\"\u003e\n\u003cth style=\"text-align: left; width: 39.9652%; height: 19.5938px;\"\u003eShipping\u003c\/th\u003e\n\u003ctd style=\"width: 59.9479%; height: 19.5938px;\"\u003eAmbient temperature\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003cth style=\"text-align: left; width: 39.9652%; height: 39.1875px;\"\u003eStorage\u003c\/th\u003e\n\u003ctd style=\"width: 59.9479%; height: 39.1875px;\"\u003eStore at 2–8 °C on receipt. Protect from light. See product data sheet for stability.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 39.1875px;\"\u003e\n\u003cth style=\"text-align: left; width: 39.9652%; height: 39.1875px;\"\u003eRegulatory\u003c\/th\u003e\n\u003ctd style=\"width: 59.9479%; height: 39.1875px;\"\u003eFor Research Use Only (RUO). Not for diagnostic use.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion\"\u003e\n\u003cdetails open=\"\"\u003e\n\u003csummary\u003eDownloads\u003cspan class=\"icon-caret\"\u003e\u003csvg viewbox=\"0 0 10 6\" class=\"icon icon-caret\"\u003e\u003cpath clip-rule=\"evenodd\" d=\"M9.354.646a.5.5 0 0 0-.708 0L5 4.293 1.354.646a.5.5 0 0 0-.708.708l4 4a.5.5 0 0 0 .708 0l4-4a.5.5 0 0 0 0-.708\" fill-rule=\"evenodd\" fill=\"currentColor\"\u003e\u003c\/path\u003e\u003c\/svg\u003e\u003c\/span\u003e\u003c\/summary\u003e\n\u003cdiv class=\"accordion__content\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/details\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"accordion\"\u003e\n\u003cdetails open=\"\"\u003e\n\u003csummary\u003ePublications\u003cspan class=\"icon-caret\"\u003e\u003csvg viewbox=\"0 0 10 6\" class=\"icon icon-caret\"\u003e\u003cpath clip-rule=\"evenodd\" d=\"M9.354.646a.5.5 0 0 0-.708 0L5 4.293 1.354.646a.5.5 0 0 0-.708.708l4 4a.5.5 0 0 0 .708 0l4-4a.5.5 0 0 0 0-.708\" fill-rule=\"evenodd\" fill=\"currentColor\"\u003e\u003c\/path\u003e\u003c\/svg\u003e\u003c\/span\u003e\u003c\/summary\u003e\n\u003cdiv class=\"accordion__content\"\u003e\n\u003cp\u003ePublications will be listed here as they appear.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/details\u003e\n\u003c\/div\u003e","brand":"tilibit nanosystems","offers":[{"title":"Anti-Rabbit \/ Standard \/ AF647","offer_id":57215206785289,"sku":"GL-ARB-AF647-1","price":555.0,"currency_code":"EUR","in_stock":true},{"title":"Anti-Rabbit \/ Ruggedized \/ AF647","offer_id":57215212421385,"sku":"GL-ARB-AF647-R-1","price":755.0,"currency_code":"EUR","in_stock":true},{"title":"Streptavidin \/ Standard \/ AF647","offer_id":57215212454153,"sku":"GL-STR-AF647-1","price":455.0,"currency_code":"EUR","in_stock":true},{"title":"Streptavidin \/ Ruggedized \/ AF647","offer_id":57215212486921,"sku":"GL-STR-AF647-R-1","price":655.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1299\/5863\/files\/glowbuberender.png?v=1766142509","url":"https:\/\/www.tilibit.com\/products\/tiliglow","provider":"tilibit nanosystems","version":"1.0","type":"link"}