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Purification - PEG

Purification - PEG

€160.40

Summary

This purification method can be used to:

  • Remove excess staple strands
  • Exchange the solution buffer
  • Increase the structure concentration

Description

This purification method is based on entropic size-exclusion effects. Folded DNA structures above a size threshold are precipitated. Excess staple strands and DNA objects smaller than the size threshold remain in solution and are removed with the supernatant. The sample is then redissolved in the target buffer solution. The buffer can be exchanged and individually adjusted to the desired final conditions. Less than 5 % of initial excess staple strands remain after the purification. Final structure concentrations in the µM range can be achieved. The solution may contain up to 10µM of  PEG-8000 molecules after purification. Use Purification - Filter purification or Purification - Gel purification if presence of PEG-8000 is not acceptable for your application.

Default conditions are 20 nM final structure concentration, dissolved in tilibit 1x folding buffer containing 5 mM MgCl2. We offer a guaranteed yield of 90% for prefabricated designs under default conditions. Final solution buffer and structure concentration may be adjusted for the desired purpose. Please contact us if you are interested in custom sample conditions.

Specifications

Guaranteed yield: 90 %

Final structure concentration: 20 nM

Final solution buffer

  • 5 mM TRIS-BASE
  • 1 mM EDTA
  • 5 mM NaCl
  • 5 mM MgCl2

Sample can contain up to 10 µM PEG-8000

 

This protocol is based on the following publication:

E. Stahl, T. Martin, F. Praetorius, and H. Dietz, 'Facile and scalable preparation of pure and dense DNA origami solutions', Angewandte Chemie Intl. Edn., vol 53 (2014), p12735



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