Purification - Gel
This purification method can be used to:
- Remove excess staple strands from well folded DNA-origami objects
- Purify a single target structure species - e.g. Separate the monomeric species from the dimeric species
Electrophoretic separation via an agarose gel matrix is a reliable method for the purification of a single target species of DNA origami structures. Excess staple strands as well as misfolded or aggregated DNA origami structures can be removed. Well-folded DNA origami structures are selected based on their increased migration speed relative to misfolded structures. The target species band is cut out from the gel and then further separated from the agarose pulp.
Average final structure concentration is 10 nM, but can vary with each Lot. We offer a guaranteed yield of 20% for prefabricated designs under default conditions. Structures are dissolved in dissolved in a buffer containing 44.5 mM Tris, 44.5 mM boric acid, 1 mM EDTA and 5 mM MgCl2. This purification method can be combined with other purification methods in order to exchange the solution buffer or to increase the structure concentration. Please contact us if you are interested in a custom sequence of multiple purifications.
Guaranteed yield: 20%
Final structure concentration: Variable, average of 10 nM
Final solution buffer
- 44.5 mM Tris
- 44.5 mM Boric acid
- 1 mM EDTA
- 5 mM MgCl2